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Genetic Chemistry
Polymerase Chain Reaction (PRC)
Polymerase Chain Reaction (PCR)
Polymerase Chain Reaction (PCR) is a widely used molecular biology technique for amplifying DNA sequences. In PCR experiments, the quality of water is crucial for various steps, including template preparation, primer and nucleotide solution preparation, and the PCR reaction itself. High-purity water is essential to prevent interference with DNA amplification and ensure the success of PCR experiments.
What Is The Impact Of Water In PCR?
Water quality significantly affects the success and reliability of PCR experiments. Contaminated water can introduce impurities into DNA samples, affect the fidelity of DNA polymerases, and compromise the accuracy of DNA amplification. High-purity water is necessary to maintain the integrity of DNA templates and ensure reliable PCR results.
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What Types Of Contaminants In Water Can Affect PCR?
Water impurities that can impact PCR experiments include:
Bacteria & Microorganisms
Impact:
Bacterial contamination can adversely affect PCR results.
Effect:
Unintended microorganisms may introduce variations in PCR samples, leading to inconsistencies in experimental conditions and compromising the reliability of PCR analyses. This can affect the accuracy of DNA amplification.
Endotoxins
Impact:
Endotoxins can affect the efficiency of PCR reactions.
Effect:
In PCR experiments, endotoxins may interfere with the amplification process, leading to variations in reaction efficiency and compromising the reliability of PCR results. This can affect the accuracy of DNA amplification and quantification.
Organic Compounds
Impact:
Small organic compounds in water can introduce unwanted signals in PCR assays.
Effect:
Organic impurities, such as humic acids and pesticides, may affect the purity of PCR samples. Removal of these organic compounds from PCR buffers is crucial to ensure accurate and reliable amplification results.
Ionic Contaminants
Impact:
Ionic contaminants can interfere with PCR amplification.
Effect:
Elevated levels of ions may affect the specificity and efficiency of PCR reactions, leading to variations in amplification results. Maintaining low levels of ionic contaminants is essential for the reproducibility and accuracy of PCR analyses.
Water Measurement Requirements for PCR
In PCR applications, achieving accurate and reproducible results is contingent on the quality of water used in the analysis. The selection of water is guided by the sensitivity of the PCR methods employed. For techniques demanding high specificity, such as quantitative PCR (qPCR), the use of apyrogenic, Type I ultrapure water is strongly recommended.
Water Quality Standards According to Industry Guidelines:
Adhering to these water quality standards is crucial for the success and accuracy of PCR experiments. Type I water is recommended for critical PCR applications, while Type II water may be suitable for routine experiments.
NOTES:
Applications requiring Endotoxin filtration should purchase a CLF-5000-02E Endotoxin removal final filter.
System
Label
System Depth (in)
System Height (in)
System Width (in)
Dry Weight (lbs)
Inlet Connection
System Voltage
TOC Monitoring
0.05μ Ultrafiltration
Ultraviolet Oxydation
Effluent Purity
Effluent Microorganisms
Effluent Sodium
Effluent Chloride
Effluent TOC
System Maximum
Output (lpm)
Volumetric Presets
Mounting
Laboratory Applications
CAtegory
System
Label
System Depth (in)
System Height (in)
System Width (in)
Dry Weight (lbs)
Inlet Connection
System Voltage
TOC Monitoring
0.05μ Ultrafiltration
Ultraviolet Oxydation
Effluent Purity
Effluent Microorganisms
Effluent Sodium
Effluent Chloride
Effluent TOC
System Maximum
Output (lpm)
Volumetric Presets
Mounting
Laboratory Applications
CAtegory
CLïR 3400 High Purity Lab Water System with 0.05 Micron Ultrafiltration and UV
10
25.25
21
32
⅜" O.D. Tubing
120V/60Hz
> 18 MΩ/cm
< 10 CFU / ml
< 1ppb
< 1ppb
< 5 ppb
4
Countertop, Wall Mount
DNA Sequencing, Forensics, Genetics, Molecular Biology, Polymerase Chain Reaction (PCR)
Genetic Chemistry
CLiR 5400 Ultrapure Lab Water System with TOC Monitoring, UV Disinfection & Ultrafiltration (Remote 120v)
14.4
21.6
14.7
42
⅜" O.D. Tubing
120V/60Hz
> 18 MΩ/cm
< 1 CFU/ml
< 1ppb
< 1ppb
< 1ppb
2
Wall Mount, Remote Dispense
DNA Sequencing, Forensics, Genetics, Molecular Biology, Polymerase Chain Reaction (PCR)
Genetic Chemistry
CLiR 5400 Ultrapure Lab Water System with TOC Monitoring, UV Disinfection & Ultrafiltration (Remote 220v)
14.4
21.6
14.7
42
⅜" O.D. Tubing
220V/50Hz
> 18 MΩ/cm
< 1 CFU/ml
< 1ppb
< 1ppb
< 1ppb
2
Wall Mount, Remote Dispense
DNA Sequencing, Forensics, Genetics, Molecular Biology, Polymerase Chain Reaction (PCR)
Genetic Chemistry
CLiR 5400 Ultrapure Lab Water System with TOC Monitoring, UV Disinfection & Ultrafiltration (Standard 120v)
14.4
21.6
14.7
42
⅜" O.D. Tubing
120V/60Hz
> 18 MΩ/cm
< 1 CFU/ml
< 1ppb
< 1ppb
< 1ppb
2
Countertop
DNA Sequencing, Forensics, Genetics, Molecular Biology, Polymerase Chain Reaction (PCR)
Genetic Chemistry
System
Label
System Depth (in)
System Height (in)
System Width (in)
Dry Weight (lbs)
Inlet Connection
System Voltage
TOC Monitoring
0.05μ Ultrafiltration
Ultraviolet Oxydation
Effluent Purity
Effluent Microorganisms
Effluent Sodium
Effluent Chloride
Effluent TOC
System Maximum
Output (lpm)
Volumetric Presets
Mounting
Laboratory Applications
CAtegory
CLïR 3400 High Purity Lab Water System with 0.05 Micron Ultrafiltration and UV
10
25.25
21
32
⅜" O.D. Tubing
120V/60Hz
> 18 MΩ/cm
< 10 CFU / ml
< 1ppb
< 1ppb
< 5 ppb
4
Countertop, Wall Mount
DNA Sequencing, Forensics, Genetics, Molecular Biology, Polymerase Chain Reaction (PCR)
System
Label
System Depth (in)
System Height (in)
System Width (in)
Dry Weight (lbs)
Inlet Connection
System Voltage
TOC Monitoring
0.05μ Ultrafiltration
Ultraviolet Oxydation
Effluent Purity
Effluent Microorganisms
Effluent Sodium
Effluent Chloride
Effluent TOC
System Maximum
Output (lpm)
Volumetric Presets
Mounting
Laboratory Applications
CAtegory
CLiR 5400 Ultrapure Lab Water System with TOC Monitoring, UV Disinfection & Ultrafiltration (Remote 120v)
14.4
21.6
14.7
42
⅜" O.D. Tubing
120V/60Hz
> 18 MΩ/cm
< 1 CFU/ml
< 1ppb
< 1ppb
< 1ppb
2
Wall Mount, Remote Dispense
DNA Sequencing, Forensics, Genetics, Molecular Biology, Polymerase Chain Reaction (PCR)
System
Label
System Depth (in)
System Height (in)
System Width (in)
Dry Weight (lbs)
Inlet Connection
System Voltage
TOC Monitoring
0.05μ Ultrafiltration
Ultraviolet Oxydation
Effluent Purity
Effluent Microorganisms
Effluent Sodium
Effluent Chloride
Effluent TOC
System Maximum
Output (lpm)
Volumetric Presets
Mounting
Laboratory Applications
CAtegory
CLiR 5400 Ultrapure Lab Water System with TOC Monitoring, UV Disinfection & Ultrafiltration (Remote 220v)
14.4
21.6
14.7
42
⅜" O.D. Tubing
220V/50Hz
> 18 MΩ/cm
< 1 CFU/ml
< 1ppb
< 1ppb
< 1ppb
2
Wall Mount, Remote Dispense
DNA Sequencing, Forensics, Genetics, Molecular Biology, Polymerase Chain Reaction (PCR)
System
Label
System Depth (in)
System Height (in)
System Width (in)
Dry Weight (lbs)
Inlet Connection
System Voltage
TOC Monitoring
0.05μ Ultrafiltration
Ultraviolet Oxydation
Effluent Purity
Effluent Microorganisms
Effluent Sodium
Effluent Chloride
Effluent TOC
System Maximum
Output (lpm)
Volumetric Presets
Mounting
Laboratory Applications
CAtegory
CLiR 5400 Ultrapure Lab Water System with TOC Monitoring, UV Disinfection & Ultrafiltration (Standard 120v)
14.4
21.6
14.7
42
⅜" O.D. Tubing
120V/60Hz
> 18 MΩ/cm
< 1 CFU/ml
< 1ppb
< 1ppb
< 1ppb
2
Countertop
DNA Sequencing, Forensics, Genetics, Molecular Biology, Polymerase Chain Reaction (PCR)
System
Label
System Depth (in)
System Height (in)
System Width (in)
Dry Weight (lbs)
Inlet Connection
System Voltage
TOC Monitoring
0.05μ Ultrafiltration
Ultraviolet Oxidation
Effluent Purity
Effluent Microorganisms
Effluent Sodium
Effluent Chlorides
Effluent TOC
System Maximum
Output (lpm)
Volumetric Presets
Mounting
Laboratory Applications
CAtegory
CLïR 3400 High Purity Lab Water System with 0.05 Micron Ultrafiltration and UV
10
25.25
21
32
⅜" O.D. Tubing
120V/60Hz
> 18 MΩ/cm
< 10 CFU / ml
< 1ppb
< 1ppb
< 5 ppb
4
Countertop, Wall Mount
DNA Sequencing, Forensics, Genetics, Molecular Biology, Polymerase Chain Reaction (PCR)
Genetic Chemistry
CLiR 5400 Ultrapure Lab Water System with TOC Monitoring, UV Disinfection & Ultrafiltration (Remote 120v)
14.4
21.6
14.7
42
⅜" O.D. Tubing
120V/60Hz
> 18 MΩ/cm
< 1 CFU/ml
< 1ppb
< 1ppb
< 1ppb
2
Wall Mount, Remote Dispense
DNA Sequencing, Forensics, Genetics, Molecular Biology, Polymerase Chain Reaction (PCR)
Genetic Chemistry
CLiR 5400 Ultrapure Lab Water System with TOC Monitoring, UV Disinfection & Ultrafiltration (Remote 220v)
14.4
21.6
14.7
42
⅜" O.D. Tubing
220V/50Hz
> 18 MΩ/cm
< 1 CFU/ml
< 1ppb
< 1ppb
< 1ppb
2
Wall Mount, Remote Dispense
DNA Sequencing, Forensics, Genetics, Molecular Biology, Polymerase Chain Reaction (PCR)
Genetic Chemistry
CLiR 5400 Ultrapure Lab Water System with TOC Monitoring, UV Disinfection & Ultrafiltration (Standard 120v)
14.4
21.6
14.7
42
⅜" O.D. Tubing
120V/60Hz
> 18 MΩ/cm
< 1 CFU/ml
< 1ppb
< 1ppb
< 1ppb
2
Countertop
DNA Sequencing, Forensics, Genetics, Molecular Biology, Polymerase Chain Reaction (PCR)
Genetic Chemistry
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