Genetic Chemistry

DNA Sequencing

DNA sequencing is a fundamental technique in genetics, enabling the determination of the nucleotide sequence of DNA molecules. In DNA sequencing experiments, the quality of water is crucial for various steps, including DNA template preparation, PCR, and the sequencing reaction itself. High-purity water is essential to prevent interference with DNA samples and ensure the accuracy of sequencing results.

What Is The Impact Of Water In DNA Sequencing?

Water quality directly influences the success and accuracy of DNA sequencing experiments. Contaminated water can introduce impurities into DNA samples, affect enzymatic reactions in PCR, and compromise the integrity of sequencing reactions. High-purity water is necessary to maintain the quality of DNA templates and ensure reliable DNA sequencing results.

What Types Of Contaminants In Water Can Affect DNA Sequencing?

Water impurities that can impact DNA sequencing experiments include:

Bacteria & Microorganisms

Impact:

Bacterial contamination can compromise the accuracy of DNA sequencing.

Effect:

Unintended microorganisms may introduce variations in DNA samples, leading to inconsistencies in experimental conditions and compromising the reliability of DNA sequencing. This can affect the accuracy of nucleotide identification.

Endotoxins

Impact:

Endotoxins can affect the quality of DNA sequences.

Effect:

In DNA sequencing experiments, endotoxins may lead to changes in DNA structure, affecting the accuracy of nucleotide base calling. This can compromise the reliability of DNA sequencing results.

Organic Compounds

Impact:

Small organic compounds in water can introduce unwanted signals in DNA sequencing assays.

Effect:

Organic impurities, such as humic acids and pesticides, may affect the purity of DNA samples. Removal of these organic compounds from DNA sequencing buffers is crucial to ensure accurate and reliable sequencing results.

Ionic Contaminants

Impact:

Ionic contaminants can interfere with DNA sequencing analyses.

Effect:

Elevated levels of ions may affect the stability of DNA structures during sequencing, leading to variations in sequencing results. Maintaining low levels of ionic contaminants is essential for the reproducibility and accuracy of DNA sequencing.

Water Measurement Requirements for DNA Sequencing

In DNA sequencing, the accuracy and reliability of results hinge on the quality of water utilized in the analysis. The choice of water is tailored to the sensitivity of the DNA sequencing methods employed. For high-throughput sequencing techniques requiring precision, the use of apyrogenic, ultrapure Type 1 water is strongly recommended.

Water Quality Standards According to Industry Guidelines:

Resistivity (MΩ.cm)*

>18

TOC (ppb)

<10

Filter (μm)

<0.2

Bacteria (CFU/ml)

Endotoxins (EU/ml)

<0.002

Nucleases

Not detected

Resistivity (MΩ.cm)*

TOC (ppb)

Filter (μm)

Bacteria (CFU/ml)

Endotoxins (EU/ml)

Nucleases

>18

<10

<0.2

<0.002

Not detected

Adhering to these water quality standards is crucial for the success and accuracy of DNA sequencing experiments. Type I water is recommended for critical DNA sequencing applications, while Type II water may be suitable for routine experiments.

NOTES:

Applications requiring Endotoxin filtration should purchase a CLF-5000-02E Endotoxin removal final filter.

CLS-3400

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CLïR 3400 High Purity Lab Water System with 0.05 Micron Ultrafiltration and UV

25.25

⅜" O.D. Tubing

120V/60Hz

> 18 MΩ/cm

< 10 CFU / ml

< 1ppb

< 5 ppb

Countertop, Wall Mount

DNA Sequencing, Forensics, Genetics, Molecular Biology, Polymerase Chain Reaction (PCR)

Genetic Chemistry

CLS-5400-R-1-TOC

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CLiR 5400 Ultrapure Lab Water System with TOC Monitoring, UV Disinfection & Ultrafiltration (Remote 120v)

14.4

21.6

14.7

⅜" O.D. Tubing

120V/60Hz

> 18 MΩ/cm

< 1 CFU/ml

< 1ppb

Wall Mount, Remote Dispense

DNA Sequencing, Forensics, Genetics, Molecular Biology, Polymerase Chain Reaction (PCR)

Genetic Chemistry

CLS-5400-R-2-TOC

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CLiR 5400 Ultrapure Lab Water System with TOC Monitoring, UV Disinfection & Ultrafiltration (Remote 220v)

14.4

21.6

14.7

⅜" O.D. Tubing

220V/50Hz

> 18 MΩ/cm

< 1 CFU/ml

< 1ppb

Wall Mount, Remote Dispense

DNA Sequencing, Forensics, Genetics, Molecular Biology, Polymerase Chain Reaction (PCR)

Genetic Chemistry

CLS-5400-S-1-TOC

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CLiR 5400 Ultrapure Lab Water System with TOC Monitoring, UV Disinfection & Ultrafiltration (Standard 120v)

14.4

21.6

14.7

⅜" O.D. Tubing

120V/60Hz

> 18 MΩ/cm

< 1 CFU/ml

< 1ppb

Countertop

DNA Sequencing, Forensics, Genetics, Molecular Biology, Polymerase Chain Reaction (PCR)

Genetic Chemistry

System

Label

System Depth (in)

System Height (in)

System Width (in)

Dry Weight (lbs)

Inlet Connection

System Voltage

TOC Monitoring

0.05μ Ultrafiltration

Ultraviolet Oxidation

Effluent Purity

Effluent Microorganisms

Effluent Sodium

Effluent Chlorides

Effluent TOC

System Maximum
Output (lpm)

Volumetric Presets

Mounting

Laboratory Applications

CAtegory

CLS-3400

Get a quote

Download Spec Sheet

CLïR 3400 High Purity Lab Water System with 0.05 Micron Ultrafiltration and UV

25.25

⅜" O.D. Tubing

120V/60Hz

> 18 MΩ/cm

< 10 CFU / ml

< 1ppb

< 5 ppb

Countertop, Wall Mount

DNA Sequencing, Forensics, Genetics, Molecular Biology, Polymerase Chain Reaction (PCR)

Genetic Chemistry

CLS-5400-R-1-TOC

Get a quote

Download Spec Sheet

CLiR 5400 Ultrapure Lab Water System with TOC Monitoring, UV Disinfection & Ultrafiltration (Remote 120v)

14.4

21.6

14.7

⅜" O.D. Tubing

120V/60Hz

> 18 MΩ/cm

< 1 CFU/ml

< 1ppb

Wall Mount, Remote Dispense

DNA Sequencing, Forensics, Genetics, Molecular Biology, Polymerase Chain Reaction (PCR)

Genetic Chemistry

CLS-5400-R-2-TOC

Get a quote

Download Spec Sheet

CLiR 5400 Ultrapure Lab Water System with TOC Monitoring, UV Disinfection & Ultrafiltration (Remote 220v)

14.4

21.6

14.7

⅜" O.D. Tubing

220V/50Hz

> 18 MΩ/cm

< 1 CFU/ml

< 1ppb

Wall Mount, Remote Dispense

DNA Sequencing, Forensics, Genetics, Molecular Biology, Polymerase Chain Reaction (PCR)

Genetic Chemistry

CLS-5400-S-1-TOC

Get a quote

Download Spec Sheet

CLiR 5400 Ultrapure Lab Water System with TOC Monitoring, UV Disinfection & Ultrafiltration (Standard 120v)

14.4

21.6

14.7

⅜" O.D. Tubing

120V/60Hz

> 18 MΩ/cm

< 1 CFU/ml

< 1ppb

Countertop

DNA Sequencing, Forensics, Genetics, Molecular Biology, Polymerase Chain Reaction (PCR)

Genetic Chemistry

The difference is CLïR.

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Applications

Genetic Chemistry

DNA Sequencing

What Is The Impact Of Water In DNA Sequencing?

Bacteria & Microorganisms

Endotoxins

Organic Compounds

Ionic Contaminants

Water Measurement Requirements for DNA Sequencing

Resistivity (MΩ.cm)*

TOC (ppb)

Filter (μm)

Bacteria (CFU/ml)

Endotoxins (EU/ml)

Nucleases

System

Label

System Depth (in)

System Height (in)

System Width (in)

Dry Weight (lbs)

Inlet Connection

System Voltage

TOC Monitoring

0.05μ Ultrafiltration

Ultraviolet Oxydation

Effluent Purity

Effluent Microorganisms

Effluent Sodium

Effluent Chloride

Effluent TOC

System MaximumOutput (lpm)

Volumetric Presets

Mounting

Laboratory Applications

CAtegory

System

Label

System Depth (in)

System Height (in)

System Width (in)

Dry Weight (lbs)

Inlet Connection

System Voltage

TOC Monitoring

0.05μ Ultrafiltration

Ultraviolet Oxydation

Effluent Purity

Effluent Microorganisms

Effluent Sodium

Effluent Chloride

Effluent TOC

System MaximumOutput (lpm)

Volumetric Presets

Mounting

Laboratory Applications

CAtegory

System

Label

System Depth (in)

System Height (in)

System Width (in)

Dry Weight (lbs)

Inlet Connection

System Voltage

TOC Monitoring

0.05μ Ultrafiltration

Ultraviolet Oxydation

Effluent Purity

Effluent Microorganisms

Effluent Sodium

Effluent Chloride

Effluent TOC

System MaximumOutput (lpm)

Volumetric Presets

Mounting

Laboratory Applications

Laboratory Applications

CAtegory

System

System Maximum
Output (lpm)

System Maximum
Output (lpm)

System Maximum
Output (lpm)

System Maximum
Output (lpm)

System Maximum
Output (lpm)

System Maximum
Output (lpm)