Genetic Chemistry

Molecular Biology

Molecular biology involves the study of biological molecules, including DNA, RNA, and proteins, to understand cellular processes and genetic information. In molecular biology experiments, the quality of water is crucial for various applications such as nucleic acid and protein purification, enzyme reactions, and hybridization. High-purity water is essential to prevent interference with molecular processes and ensure the success of molecular biology experiments.

What Is The Impact Of Water In
Molecular Biology?

Water quality plays a critical role in the success of molecular biology experiments. Contaminated water can introduce impurities into samples, affect enzymatic reactions, and compromise the accuracy of molecular analyses. High-purity water is necessary to maintain the integrity of nucleic acids and proteins, ensuring reliable results in molecular biology experiments.

What Types Of Contaminants In Water Can Affect Molecular Biology?

Water impurities that can impact molecular biology experiments include:

Bacteria & Microorganisms

Impact:

Bacterial contamination can adversely affect molecular biology experiments.

Effect:

Unintended microorganisms may introduce variations in molecular samples, leading to inconsistencies in experimental conditions and compromising the reliability of molecular biology analyses. This can affect the accuracy of DNA, RNA, or protein studies.

Endotoxins

Impact:

Endotoxins can affect the quality of molecular samples.

Effect:

In molecular biology experiments, endotoxins may lead to changes in sample characteristics, affecting the accuracy of nucleic acid or protein studies. This can compromise the reliability of molecular biology results.

Organic Compounds

Impact:

Small organic compounds in water can introduce unwanted signals in molecular biology assays.

Effect:

Organic impurities, such as humic acids and pesticides, may affect the purity of molecular samples. Removal of these organic compounds from molecular biology buffers is crucial to ensure accurate and reliable molecular biology results.

Ionic Contaminants

Impact:

Ionic contaminants can interfere with molecular biology analyses.

Impact:

Elevated levels of ions may affect the stability and characteristics of molecular samples, leading to variations in molecular biology results. Maintaining low levels of ionic contaminants is essential for the reproducibility and accuracy of molecular biology analyses.

Water Measurement Requirements for Molecular Biology

In molecular biology applications, the accuracy and reliability of experimental outcomes heavily rely on the quality of water utilized. The choice of water is tailored to the sensitivity of molecular biology methods employed. For techniques demanding high precision, such as DNA amplification and molecular cloning, the use of apyrogenic, Type I ultrapure water is strongly recommended.

Water Quality Standards According to Industry Guidelines:

Resistivity (MΩ.cm)*

>18

TOC (ppb)

<10

Filter (μm)

<0.2

Bacteria (CFU/ml)

Endotoxins (EU/ml)

<0.002

Nucleases

Not detected

Application

Resistivity (MΩ.cm)*

TOC (ppb)

Filter (μm)

Bacteria (CFU/ml)

Endotoxins (EU/ml)

Nucleases

Molecular Biology

>18

<10

<0.2

<0.002

Not Detected

Adhering to these water quality standards is crucial for the success and accuracy of molecular biology experiments. Type I water is recommended for critical applications, while Type II water may be suitable for routine experiments.

NOTES:

Applications requiring Endotoxin filtration should purchase a CLF-5000-02E Endotoxin removal final filter.

CLS-3400

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CLïR 3400 High Purity Lab Water System with 0.05 Micron Ultrafiltration and UV

25.25

⅜" O.D. Tubing

120V/60Hz

> 18 MΩ/cm

< 10 CFU / ml

< 1ppb

< 5 ppb

Countertop, Wall Mount

DNA Sequencing, Forensics, Genetics, Molecular Biology, Polymerase Chain Reaction (PCR)

Genetic Chemistry

CLS-5400-R-1-TOC

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CLiR 5400 Ultrapure Lab Water System with TOC Monitoring, UV Disinfection & Ultrafiltration (Remote 120v)

14.4

21.6

14.7

⅜" O.D. Tubing

120V/60Hz

> 18 MΩ/cm

< 1 CFU/ml

< 1ppb

Wall Mount, Remote Dispense

DNA Sequencing, Forensics, Genetics, Molecular Biology, Polymerase Chain Reaction (PCR)

Genetic Chemistry

CLS-5400-R-2-TOC

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CLiR 5400 Ultrapure Lab Water System with TOC Monitoring, UV Disinfection & Ultrafiltration (Remote 220v)

14.4

21.6

14.7

⅜" O.D. Tubing

220V/50Hz

> 18 MΩ/cm

< 1 CFU/ml

< 1ppb

Wall Mount, Remote Dispense

DNA Sequencing, Forensics, Genetics, Molecular Biology, Polymerase Chain Reaction (PCR)

Genetic Chemistry

CLS-5400-S-1-TOC

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CLiR 5400 Ultrapure Lab Water System with TOC Monitoring, UV Disinfection & Ultrafiltration (Standard 120v)

14.4

21.6

14.7

⅜" O.D. Tubing

120V/60Hz

> 18 MΩ/cm

< 1 CFU/ml

< 1ppb

Countertop

DNA Sequencing, Forensics, Genetics, Molecular Biology, Polymerase Chain Reaction (PCR)

Genetic Chemistry

System

Label

System Depth (in)

System Height (in)

System Width (in)

Dry Weight (lbs)

Inlet Connection

System Voltage

TOC Monitoring

0.05μ Ultrafiltration

Ultraviolet Oxidation

Effluent Purity

Effluent Microorganisms

Effluent Sodium

Effluent Chlorides

Effluent TOC

System Maximum
Output (lpm)

Volumetric Presets

Mounting

Laboratory Applications

CAtegory

CLS-3400

Get a quote

Download Spec Sheet

CLïR 3400 High Purity Lab Water System with 0.05 Micron Ultrafiltration and UV

25.25

⅜" O.D. Tubing

120V/60Hz

> 18 MΩ/cm

< 10 CFU / ml

< 1ppb

< 5 ppb

Countertop, Wall Mount

DNA Sequencing, Forensics, Genetics, Molecular Biology, Polymerase Chain Reaction (PCR)

Genetic Chemistry

CLS-5400-R-1-TOC

Get a quote

Download Spec Sheet

CLiR 5400 Ultrapure Lab Water System with TOC Monitoring, UV Disinfection & Ultrafiltration (Remote 120v)

14.4

21.6

14.7

⅜" O.D. Tubing

120V/60Hz

> 18 MΩ/cm

< 1 CFU/ml

< 1ppb

Wall Mount, Remote Dispense

DNA Sequencing, Forensics, Genetics, Molecular Biology, Polymerase Chain Reaction (PCR)

Genetic Chemistry

CLS-5400-R-2-TOC

Get a quote

Download Spec Sheet

CLiR 5400 Ultrapure Lab Water System with TOC Monitoring, UV Disinfection & Ultrafiltration (Remote 220v)

14.4

21.6

14.7

⅜" O.D. Tubing

220V/50Hz

> 18 MΩ/cm

< 1 CFU/ml

< 1ppb

Wall Mount, Remote Dispense

DNA Sequencing, Forensics, Genetics, Molecular Biology, Polymerase Chain Reaction (PCR)

Genetic Chemistry

CLS-5400-S-1-TOC

Get a quote

Download Spec Sheet

CLiR 5400 Ultrapure Lab Water System with TOC Monitoring, UV Disinfection & Ultrafiltration (Standard 120v)

14.4

21.6

14.7

⅜" O.D. Tubing

120V/60Hz

> 18 MΩ/cm

< 1 CFU/ml

< 1ppb

Countertop

DNA Sequencing, Forensics, Genetics, Molecular Biology, Polymerase Chain Reaction (PCR)

Genetic Chemistry

The difference is CLïR.

Get a quote

Applications

Genetic Chemistry

Molecular Biology

What Is The Impact Of Water InMolecular Biology?

Bacteria & Microorganisms

Endotoxins

Organic Compounds

Ionic Contaminants

Water Measurement Requirements for Molecular Biology

Resistivity (MΩ.cm)*

TOC (ppb)

Filter (μm)

Bacteria (CFU/ml)

Endotoxins (EU/ml)

Nucleases

System

Label

System Depth (in)

System Height (in)

System Width (in)

Dry Weight (lbs)

Inlet Connection

System Voltage

TOC Monitoring

0.05μ Ultrafiltration

Ultraviolet Oxydation

Effluent Purity

Effluent Microorganisms

Effluent Sodium

Effluent Chloride

Effluent TOC

System MaximumOutput (lpm)

Volumetric Presets

Mounting

Laboratory Applications

CAtegory

System

Label

System Depth (in)

System Height (in)

System Width (in)

Dry Weight (lbs)

Inlet Connection

System Voltage

TOC Monitoring

0.05μ Ultrafiltration

Ultraviolet Oxydation

Effluent Purity

Effluent Microorganisms

Effluent Sodium

Effluent Chloride

Effluent TOC

System MaximumOutput (lpm)

Volumetric Presets

Mounting

Laboratory Applications

CAtegory

System

Label

System Depth (in)

System Height (in)

System Width (in)

Dry Weight (lbs)

Inlet Connection

System Voltage

TOC Monitoring

0.05μ Ultrafiltration

Ultraviolet Oxydation

Effluent Purity

Effluent Microorganisms

Effluent Sodium

Effluent Chloride

Effluent TOC

System MaximumOutput (lpm)

Volumetric Presets

Mounting

Laboratory Applications

Laboratory Applications

CAtegory

System

What Is The Impact Of Water In
Molecular Biology?

System Maximum
Output (lpm)

System Maximum
Output (lpm)

System Maximum
Output (lpm)

System Maximum
Output (lpm)

System Maximum
Output (lpm)

System Maximum
Output (lpm)