Biologic Chemistry

Microbiology

Microbiology involves the study of microorganisms, including bacteria, viruses, fungi, and parasites. In microbiological research and testing, the quality of water is paramount for various applications such as media preparation, dilution, and culture. High-purity water is essential to prevent contamination and ensure the reliability of results in microbiological experiments.

What Is The Impact Of Water In Microbiology?

Water quality plays a critical role in microbiology. Contaminated water can introduce unwanted microorganisms, affect the composition of culture media, and compromise the accuracy of microbiological tests. High-purity water is necessary to maintain the integrity of microbiological experiments and prevent unintended contamination.

What Types Of Contaminants In Water Can Affect Microbiology?

Water impurities that can impact microbiological experiments include:

Bacteria & Microorganisms

Impact:

Bacterial contamination can compromise the integrity of microbiological experiments.

Effect:

Unintended microorganisms can alter the composition of microbiological samples, leading to inconsistencies in experimental conditions and compromising the reliability of microbiological studies.

Endotoxins

Impact:

Endotoxins released by bacteria can affect microbial cultures.

Effect:

In microbiological experiments, endotoxins may stimulate adverse microbial responses, affecting the growth and function of microorganisms. This can lead to inaccuracies in microbial studies and compromise the reliability of results.

Organic Compounds

Impact:

Small organic compounds in water can affect microbial development.

Effect:

Organic impurities, such as humic acids and pesticides, can provide uncontrolled nutrients for microbial growth. Removal of these organic compounds from water used in microbiology is crucial to avoid adverse effects on microbial development.

Ionic Contaminants

Impact:

Ionic contaminants, particularly heavy metals, must be kept low.

Effect:

Heavy metals, known to be cytotoxic to various microorganisms, can negatively impact microbial growth. Maintaining low levels of ionic contaminants is essential to ensure the success of microbiological experiments.

Water Measurement Requirements for Microbiology

Genetic chemistry applications demand a high level of water purity to ensure the accuracy of genetic analyses. The choice of water type is driven by the sensitivity of the genetic methods employed. For techniques requiring precision and reliability, such as polymerase chain reaction (PCR) and genetic sequencing, the use of apyrogenic, ultrapure Type 1 water is strongly recommended.

Water Quality Standards According to Industry Guidelines:

Resistivity (MΩ.cm)*

>18

TOC (ppb)

<10

Filter (μm)

<0.2

Bacteria (CFU/ml)

Endotoxins (EU/ml)

<0.002

Nucleases

Not detected

Resistivity (MΩ.cm)*

TOC (ppb)

Filter (μm)

Bacteria (CFU/ml)

Endotoxins (EU/ml)

Nucleases

>18

<10

<0.2

<0.002

Not detected

Adhering to these water quality standards is crucial for the success and accuracy of genetic experiments. Type I water is recommended for critical genetic applications, while Type Il water may be suitable for routine experiments.

NOTES:

If removal of finer particulates (< 0.05 micron) is required, consider upgrading to CLS-5400 series systems.
Applications requiring Endotoxin filtration should purchase a CLF-5000-02E Endotoxin removal final filter.

CLS-3300

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CLR 3300 High Purity Lab Water System with 0.05 Micron Ultrafiltration

25.25

⅜" O.D. Tubing

120V/60Hz

> 18 MΩ/cm

< 10 CFU / ml

< 1ppb

< 15 ppb

Countertop, Wall Mount

Cell Culture, Electrophoresis, Immunochemistry, Life Sciences, Microbiology

Biologic Chemistry

CLS-5300-R-1

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CLïR 5300 Ultrapure Lab Water System with Ultrafiltration (Remote 120v)

14.4

21.6

14.7

⅜" O.D. Tubing

120V/60Hz

> 18 MΩ/cm

< 1 CFU/ml

< 1ppb

< 15 ppb

Wall Mount, Remote Dispense

Cell Culture, Electrophoresis, Immunochemistry, Life Sciences, Microbiology

Biologic Chemistry

CLS-5300-R-2

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CLïR 5300 Ultrapure Lab Water System with Ultrafiltration (Remote 220v)

14.4

21.6

14.7

⅜" O.D. Tubing

220V/50Hz

> 18 MΩ/cm

< 1 CFU/ml

< 1ppb

< 15 ppb

Wall Mount, Remote Dispense

Cell Culture, Electrophoresis, Immunochemistry, Life Sciences, Microbiology

Biologic Chemistry

CLS-5300-S-1

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Download Spec Sheet

CLïR 5300 Ultrapure Lab Water System with Ultrafiltration (Standard 120v)

14.4

21.6

14.7

⅜" O.D. Tubing

120V/60Hz

> 18 MΩ/cm

< 1 CFU/ml

< 1ppb

< 15 ppb

Countertop

Cell Culture, Electrophoresis, Immunochemistry, Life Sciences, Microbiology

Biologic Chemistry

System

Label

System Depth (in)

System Height (in)

System Width (in)

Dry Weight (lbs)

Inlet Connection

System Voltage

TOC Monitoring

0.05μ Ultrafiltration

Ultraviolet Oxidation

Effluent Purity

Effluent Microorganisms

Effluent Sodium

Effluent Chlorides

Effluent TOC

System Maximum
Output (lpm)

Volumetric Presets

Mounting

Laboratory Applications

The difference is CLïR.

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Applications

Biologic Chemistry

Microbiology

What Is The Impact Of Water In Microbiology?

Bacteria & Microorganisms

Endotoxins

Organic Compounds

Ionic Contaminants

Water Measurement Requirements for Microbiology

Resistivity (MΩ.cm)*

TOC (ppb)

Filter (μm)

Bacteria (CFU/ml)

Endotoxins (EU/ml)

Nucleases

System

Label

System Depth (in)

System Height (in)

System Width (in)

Dry Weight (lbs)

Inlet Connection

System Voltage

TOC Monitoring

0.05μ Ultrafiltration

Ultraviolet Oxydation

Effluent Purity

Effluent Microorganisms

Effluent Sodium

Effluent Chloride

Effluent TOC

System MaximumOutput (lpm)

Volumetric Presets

Mounting

Laboratory Applications

CAtegory

System

Label

System Depth (in)

System Height (in)

System Width (in)

Dry Weight (lbs)

Inlet Connection

System Voltage

TOC Monitoring

0.05μ Ultrafiltration

Ultraviolet Oxydation

Effluent Purity

Effluent Microorganisms

Effluent Sodium

Effluent Chloride

Effluent TOC

System MaximumOutput (lpm)

Volumetric Presets

Mounting

Laboratory Applications

CAtegory

System

Label

System Depth (in)

System Height (in)

System Width (in)

Dry Weight (lbs)

Inlet Connection

System Voltage

TOC Monitoring

0.05μ Ultrafiltration

Ultraviolet Oxidation

Effluent Purity

Effluent Microorganisms

Effluent Sodium

Effluent Chlorides

Effluent TOC

System MaximumOutput (lpm)

Volumetric Presets

Mounting

Laboratory Applications

CAtegory

System

Label

System Maximum
Output (lpm)

System Maximum
Output (lpm)

System Maximum
Output (lpm)

System Maximum
Output (lpm)

System Maximum
Output (lpm)

System Maximum
Output (lpm)